DESCRIPTION
The Quick-DNA Fungal/Bacterial Miniprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
| Applicable For |
All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume |
≥ 35 µl |
| Equipment |
Microcentrifuge, vortex, cell disruptor/pulverizer |
| Processing Time |
≤ 15 minutes |
| Processing Volume |
≤100 mg fungi or bacteria (wet weight), 109 bacterial cells, 108 yeast cells, or 107 mammalian cells |
| Purity |
Typical A260/A280 & A260/A230 ≥ 1.8 |
| Sample Source |
Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| Size Range |
Capable of recovering genomic DNA sized fragments from up to and above 40 kb. Typical fragment sizes range from 25 kb – 35 kb. If present, parasitic and viral DNA will also be recovered |
| Type |
Total DNA |
| Yield |
≤ 25 µg total DNA |
| Supplemental Info |
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