DESCRIPTION

The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.

TECHNICAL SPECIFICATIONS

Applicable For	All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume	≥ 50 µl
Equipment	Microcentrifuge, vortex, cell disruptor/pulverizer
Processing Volume	≤ 150 mg
Purity	A260/A280 nm ≥1.8.
Sample Source	Leaves, stems, buds, flowers, fruit, seeds, etc.
Sample Storage	DNA stored at ≤ -20°C.
Size Range	Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb – 35 kb. If present, parasitic and viral DNA will also be recovered.
Type	Total DNA
Yield	≤ 25 µg total DNA
Supplemental Info	Purpose of Genomic DNA Extraction & Tips For Success